Close the tube lid and put the tube on ice. Count how many colonies are present on each plate. For example, bacterial antibiotic resistance genes are often carried on plasmids. The factors involved in this cost and its potential compensation remain largely unknown.
Plasmids and chromosomes are replicated using the same enzymes, but plasmids are replicated and inherited independently from the bacterial chromosomes.
Were your results different from what you expected?
The transformed bacterial colonies glow green when illuminated with the UV light. The website below provides information and pictures of the transformation and regeneration of a tomato explant using Agrobacterium tumefaciens to carry the engineered DNA into the tomato cell.
Plasmids and their usefulness in molecular cloning.
Which one is it?